BIOL 2230

BIOL 2230

Introduction to Microbiology with Laboratory
4 Credit Hours

Course Information

Course Description:
This combination laboratory and lecture course consists of a survey of microorganisms with emphasis on morphology, growth and pathogenesis of bacteria, fungi and viruses. Human defense mechanisms against disease are emphasized. Laboratory exercises are designed to demonstrate the distribution, isolation, cultivation, identification, and control of microorganisms. The use of microorganisms in biotechnology will be examined.
Learning Outcomes: After completing this course, the student will be able to: 1. Discuss the significance of microbiology in our daily life. 2. Identify the contributions to microbiology made by early microbiologists such as Leeuwenhoek, Pasteur, Koch, Lister, Ehrlich and Jenner. 3. Compare the theories of spontaneous generation and biogenesis. 4. Recognize scientific names of organisms. 5. List the major groups of organisms studied in microbiology. 6. Cite advantages of darkfield, phase-contrast, and fluorescence microscopy. 7. Identify the characteristics of Domains and other classifications of microorganisms. 8. List the defining characteristics of the four phyla of fungi. 9. Describe the chemical composition of a typical virus. 10. Explain how viruses are cultured. 11. Compare and contrast the multiplication cycle of DNA and RNA viruses. 12. Define the key terms related to the destruction or suppression of microbial growth: sterilization, disinfection, antisepsis, germicide, bacteriostasis, asepsis, degerming. 13. Describe physical and chemical methods of microbial control. 14. List Koch's postulates. 15. Define nosocomial infections and explain their importance. 16. Define pathogen, etiology, infection, host, disease, acute, chronic and latent. 17. Define pathogenicity and virulence. 18. Describe proper sterile technique for handling microorganisms. 19. Learn the parts and correct method for use of a standard laboratory light microscope equipped with an oil immersion objective. 20. Use proper techniques for preparing and heat fixing a smear and staining bacteria. 21. Carry out and interpret simple, differential and special stains. 22. Compare and contrast the cell walls of Gram-positive and Gram negative bacteria. 23. Understand and interpret various biochemical tests for identifying bacteria. 24. Identify various protozoa, fungi, and extracellular parasites and list diseases caused. 25. Describe and demonstrate the various methods used for control of microbial growth and evaluation of disinfectants and antibiotics. 26. Understand the theory behind and procedure for basic immunological techniques. 27. Know growth characteristics and tests for identifying various bacterial groups. 28. Carry out basic procedures used to determine water purity. 29. Explain how capsules, enzymes, and toxins contribute to pathogenicity. 30. Contrast the nature and effects of exotoxins and endotoxins. 31. Contrast the four kinds of acquired immunity. 32. Explain what an antibody is and describe its structural and chemical characteristics 33. Describe the types of T cells. 34. Define a chemotherapeutic agent and distinguish between a synthetic drug and an antibiotic. 35. Describe the methods of action of each of the commonly used antibacterial drugs. 36. Discuss the causative agent, symptoms, methods of transmission and treatment of various bacterial, viral, and fungal diseases.

Prerequisites and Corequisites:
None
Course Topics:
The Microbial World and You Observing Microorganisms Functional Anatomy Growth Control of Growth Classification The Procaryotes Fungi, Protozoans, and Multicellular Parasites Viruses Principles of Disease Pathogenicity Nonspecific Defense Specific Defense Antimicrobial Drugs Diseases of Skin and Eyes Diseases of Nervous System Disease of Cardiovascular and Lymphatic Systems Diseases of Respiratory System Diseases of Digestive System, Urinary and Reproductive Systems
Specific Course Requirements:

Textbooks, Supplementary Materials, Hardware and Software Requirements

Required Textbooks:

Please visit the Virtual Bookstore for textbook information: http://rodp.bkstr.com.

Supplementary Materials:

Hardware Requirements:

The minimum requirements can be found at http://www.rodp.org/students/hardware_software.htm. Specific hardware

requirements for this course include.

Software Requirements:

The minimum requirements can be found at http://www.rodp.org/students/hardware_software.htm. Additional

Requirements include: Microsoft Office Word, Acrobat Reader, Shockwave and PowerPoint.

In addition each student will need Virtual Unknown Student software.

Instructor Information

Please see the separate page inside the course to find instructor contact information

as well as a statement of virtual office hours and other communication information.

Assessment and Grading

Testing Procedures:

Testing will be done online during a specified time period.

Grading Procedure:

A student’s grade will be determined from a total of points obtained from 4 sources: (1) laboratory tests;

(2) lecture tests and final examination; (3) laboratory work ; (4) assignments. Points will be distributed as follows:

5 Lecture tests = 500 points

Lecture Assignments = 100 points

1 Comprehensive final examination = 100 points

2 Papers = 200 points

2 Laboratory Tests = 200 points

2 Virtual Unknowns = 200 points

Laboratory Assignments = 100 points

Total = 1400 points

Grading Scale:

FINAL GRADE =        90% of 1400 = A = 1260 – 1400 points
                                    80% of 1400 = B = 1120 - 1259 points
                                    70% of 1400 = C =   980 – 1119 points
                                    60% of 1400 = D =   840 –   979 points
                                    Below 60%    = F   = less than 840 points

Assignments and Participation

Assignments and Projects:

The course will include a combination of tests, quizzes, assignments and two papers.

A number of virtual laboratory experiments will be performed.

The student will complete one module in each approximately 2.5 -3 week period in the order

shown below.

Module 1

The Microbial World and You

Observing Microorganisms

Functional Anatomy

Growth

Laboratory Assignments:

Cell Structure

Staining Reactions

Growth Curve

Module 2

Control of Growth

Classification

The Procaryotes

Fungi, Protozoans, and Multicellular Parasites

Viruses

Laboratory Assignments:

Virtual Unknown tutorial

Biochemistry

Module 3

Principles of Disease

Pathogenicity

Nonspecific Defense

Specific Defense

Disorders

Laboratory Assignments

Serology Laboratory - Elisa Test and Ouchterlony Tests

Virtual Unknown 1

Module 4

Antimicrobial Drugs

Diseases of Skin and Eyes

Diseases of Nervous System

Laboratory Assignments:

Virtual Unknown 2

Streptococcus, Staphylococcus and Neisseria

Module 5

Disease of Cardiovascular and Lymphatic Systems

Diseases of Respiratory System and Digestive System

Diseases of the Urinary and Reproductive Systems

Laboratory Assignments:

Fungi and Parasites

Enterics and Water

Class Participation:

To prevent a feeling of isolation students are encouraged to monitor discussions and to respond to

appeals for help and information from other students. In addition, regular contact with the instructor regarding

questions, etc. is necessary.

Punctuality:

The student is expected to do and self grade but not submit some homework assignments for each unit.

Since completing all work in a timely manner is essential to success in the course, all tests and quizzes must be

taken in the time period specified on the calendar. Failure to take a test or quiz or submit an assignment on

time will result in a 10 point penalty for each calendar day that the work is overdue. Final Exams will not be

accepted late. A student who expects to miss a deadline through illness, death in family etc. must contact the

instructor before the deadline to determine if an exception to the rule is possible.

Course Ground Rules

Exam Policy:

1. ALL exams are to be taken at times scheduled by the instructor. Exact dates for each

assignment are posted on the course calendar. It is essential that you do all assignments,

quizzes, tests, etc in a timely manner. Assignments submitted late will be given half credit.

2. ALL tests, exams and quizzes scheduled in the course by the instructor must be taken in order

for the student to receive a passing grade.

3. Make-up exams will be given for excused absences only (excused absences include illness,

death in family, and military or jury duty). Makeup exams will be different from exams taken

at scheduled times.

4. Should a make up be allowed, tests must be taken before the next scheduled test.

5. Violations of academic integrity will not be tolerated. You are responsible for making

yourself aware of and understanding the policies and procedures that pertain to Academic

and Classroom Misconduct. These policies include plagiarism, cheating and other forms

of academic dishonesty.

Guidelines for Communications

Email:

· Always include a subject line.

· Remember without facial expressions some comments may be taken the wrong way.

Be careful in wording your emails.

· Use of emoticons might be helpful in some cases.

· Use standard fonts.

· Do not send large attachments without permission.

· Special formatting such as centering, audio messages, tables, html, etc. should be

avoided unless necessary to complete an assignment or other communication.

· Respect the privacy of other class members

Discussion Groups:

· Review the discussion threads thoroughly before entering the discussion. Be a lurker

then a discussant.

· Try to maintain threads by using the "Reply" button rather starting a new topic.

· Do not make insulting or inflammatory statements to other members of the discussion

group. Be respectful of others' ideas.

· Be patient and read the comments of other group members thoroughly before entering your remarks.

· Be cooperative with group leaders in completing assigned tasks.

· Be positive and constructive in group discussions.

· Respond in a thoughtful and timely manner.

Chat:

· Introduce yourself to the other learners in the chat session.

· Be polite. Choose your words carefully. Do not use derogatory statements.

· Be concise in responding to others in the chat session.

· Be prepared to open the chat session at the scheduled time.

· Be constructive in your comments and suggestion

Web Resources:

· Columbia Guide to Online Style by Janice R. Walker and Todd Taylor

· Citation Styles Online http://www.bedfordstmartins.com/online/cite6.html

Library

The Tennessee Virtual Library is available to all students enrolled in the Regents Degree Program.

Links to library materials (such as electronic journals, databases, interlibrary loans, digital reserves,

dictionaries, encyclopedias, maps, and librarian support) and Internet resources needed by learners to

complete online assignments and as background reading must be included in all courses.

Students With Disabilities

Qualified students with disabilities will be provided reasonable and necessary academic accommodations

if determined eligible by the appropriate disability services staff at their home institution. Prior to granting

disability accommodations in this course, the instructor must receive written verification of a student's eligibility

for specific accommodations from the disability services staff at the home institution. It is the student's responsibility

to initiate contact with their home institution's disability services staff and to follow the established procedures for

having the accommodation notice sent to the instructor.

Syllabus Changes

The instructor reserves the right to make changes as necessary to this syllabus. If changes are necessitated

during the term of the course, the instructor will immediately notify students of such changes both by

individual email communication and posting both notification and nature of change(s) on the course bulletin board.

Technical Support

Telephone Support:

If you are having problems logging into your course,
timing out of your course, using your course web site tools, or other technical problems, please contact the AskRODP

Help Desk by calling 1-866-550-7637 (toll free)

or go to the AskRODP website at:

http://help.rodp.org